am630 cb2 receptor antagonist Search Results


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Δ 9 -THC toxicity in HL-1 cells involves cytoplasmic vacuolization. (A) Δ 9 -THC and/or ethanol decreases cell viability. HL-1 cells were treated with the indicated concentrations of Δ 9 -THC and/or ethanol for 48 h. Cellular viabilities were determined by modified MTT assay. Mean cell viability of control cells [Δ 9 -THC (-), ethanol (-)] was set to 100 %, and relative cell viabilities of Δ 9 -THC and/or ethanol treated cells are shown [mean ± S.D. (n = 6); **, p < 0.01]. (B) Cells were treated with the indicated concentrations of Δ 9 -THC and/or ethanol for 48 h and observed under light microscopy. (C) Δ 9 -THC toxicity is not ameliorated by CB-R1/2 antagonists. Cells were pretreated with 1 μM <t>AM630</t> or 1 μM AM251 for 1 h and further treated with the indicated concentrations of Δ 9 -THC and/or ethanol for 48 h. Graph shows the result of modified MTT assay [mean ± S.D. (n = 6); **, p < 0.01 versus untreated group].
Am630, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Δ 9 -THC toxicity in HL-1 cells involves cytoplasmic vacuolization. (A) Δ 9 -THC and/or ethanol decreases cell viability. HL-1 cells were treated with the indicated concentrations of Δ 9 -THC and/or ethanol for 48 h. Cellular viabilities were determined by modified MTT assay. Mean cell viability of control cells [Δ 9 -THC (-), ethanol (-)] was set to 100 %, and relative cell viabilities of Δ 9 -THC and/or ethanol treated cells are shown [mean ± S.D. (n = 6); **, p < 0.01]. (B) Cells were treated with the indicated concentrations of Δ 9 -THC and/or ethanol for 48 h and observed under light microscopy. (C) Δ 9 -THC toxicity is not ameliorated by CB-R1/2 antagonists. Cells were pretreated with 1 μM AM630 or 1 μM AM251 for 1 h and further treated with the indicated concentrations of Δ 9 -THC and/or ethanol for 48 h. Graph shows the result of modified MTT assay [mean ± S.D. (n = 6); **, p < 0.01 versus untreated group].

Journal: Toxicology Reports

Article Title: Possible roles of AMPK and macropinocytosis in the defense responses against Δ 9 -THC toxicity on HL-1 cardiomyocytes

doi: 10.1016/j.toxrep.2021.04.014

Figure Lengend Snippet: Δ 9 -THC toxicity in HL-1 cells involves cytoplasmic vacuolization. (A) Δ 9 -THC and/or ethanol decreases cell viability. HL-1 cells were treated with the indicated concentrations of Δ 9 -THC and/or ethanol for 48 h. Cellular viabilities were determined by modified MTT assay. Mean cell viability of control cells [Δ 9 -THC (-), ethanol (-)] was set to 100 %, and relative cell viabilities of Δ 9 -THC and/or ethanol treated cells are shown [mean ± S.D. (n = 6); **, p < 0.01]. (B) Cells were treated with the indicated concentrations of Δ 9 -THC and/or ethanol for 48 h and observed under light microscopy. (C) Δ 9 -THC toxicity is not ameliorated by CB-R1/2 antagonists. Cells were pretreated with 1 μM AM630 or 1 μM AM251 for 1 h and further treated with the indicated concentrations of Δ 9 -THC and/or ethanol for 48 h. Graph shows the result of modified MTT assay [mean ± S.D. (n = 6); **, p < 0.01 versus untreated group].

Article Snippet: Ethanol, AM251, and AM630 were purchased from FUJIFILM Wako Pure Chemical.

Techniques: Modification, MTT Assay, Light Microscopy